abstract：:Presented in this short communication are design principles for the construction of a positioning apparatus for biological specimens in magnetically sensitive environments. An apparatus with 3 degrees of freedom was built and found to provide position accuracy to within +/- 0.25 mm throughout a cubic volume measuring ...

journal_title：Journal of neuroscience methods

authors： MacHattie DB,Pengelly LD

更新日期：1992-08-01 00:00:00

abstract：:This paper describes the design and utility of a perfusion controller to facilitate the handling of multiple extracellular solutions. The controller consists of an interface box with both manual and computer controls and a set of solenoid valves. This provides remote handling of the switching of multiple solutions int...

journal_title：Journal of neuroscience methods

authors： Stimers JR

更新日期：1992-07-01 00:00:00

abstract：:Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...

journal_title：Journal of neuroscience methods

authors： Reinis S,Weiss DS,McGaraughty S,Tsoukatos J

更新日期：1992-06-01 00:00:00

abstract：:The electrochemical technique of differential pulse voltammetry (DPV) with micro-biosensors has been used for a number of years to monitor in vivo and in situ changes in the extracellular concentration of cerebral ascorbic acid, as well as that of the metabolites of dopamine (DA) and serotonin (5-HT). We have recently...

journal_title：Journal of neuroscience methods

authors： Crespi F,Möbius C

更新日期：1992-05-01 00:00:00

abstract：:In this report we describe the development of an in vitro preparation of the mudpuppy (Necturus maculatus) used to investigate locomotion in walking vertebrates. The preparation consists of the first 5 segments of the cervical spinal cord and the attached forelimb. The preparation is bathed in a cooled (15 degrees C) ...

journal_title：Journal of neuroscience methods

authors： Wheatley M,Stein RB

更新日期：1992-04-01 00:00:00

abstract：:We describe an immunocytochemical method for marking microelectrode tracks made during single-unit recordings in long surviving, awake monkeys. This procedure detects the increase in glial fibrillary acidic protein in the glial cells along a microelectrode track using commercially available antibodies. We have success...

journal_title：Journal of neuroscience methods

authors： Benevento LA,McCleary LB

更新日期：1992-03-01 00:00:00

abstract：:A Guard-PAK precolumn system was used for the reversed-phase liquid chromatography purification of a small, synthetic radiolabeled opioid peptide, FK 33-824 (D-Ala2, methyl-phe4, Met (O)ol5 enkephalin) (FK). This procedure involves trace enrichment of iodinated peptide onto the precolumn while iodination reagents are ...

journal_title：Journal of neuroscience methods

authors： Miller MM,Gould BE,Joshi D,Bennett HP,James S,Billiar RB

更新日期：1992-02-01 00:00:00

abstract：:A rapid, sensitive, non-isotopic in situ hybridization histochemistry protocol is presented to study the expression of mRNA at the single cell level in anatomically complex structures of the mammalian central nervous system. The protocol uses digoxigenin-UTP-labeled riboprobes, freefloating sections, and alkaline phos...

journal_title：Journal of neuroscience methods

authors： Wahle P,Beckh S

更新日期：1992-02-01 00:00:00

abstract：:The biotin derivative, N-(2-aminoethyl) biotinamide hydrochloride, or Neurobiotin, has been shown recently to be a useful marker for intracellular and anterograde tracing. The properties of Neurobiotin as a tracer were further examined in this study by making pressure injections into different regions of the cerebral ...

journal_title：Journal of neuroscience methods

authors： Huang Q,Zhou D,DiFiglia M

更新日期：1992-01-01 00:00:00

abstract：:Glial fibrillary acidic protein (GFAP) was purified from normal bovine brain by a modification of the procedure used to isolate vimentin in order to avoid contamination by other cytoskeletal components: vimentin, neurofilament triplet proteins, tubulin and actin. GFAP is thought to be separated from vimentin in the DE...

journal_title：Journal of neuroscience methods

authors： Fukuyama R,Fushiki S,Fujita S

更新日期：1991-12-01 00:00:00

abstract：:New conditions for quantitative autoradiographic determination of natriuretic peptide (ANP) binding in rat brain were developed in order to inhibit hormone degradation during incubation without the use of proteinase inhibitors. This procedure includes 1-h preincubation of tissue slices in hypotonic buffer and incubati...

journal_title：Journal of neuroscience methods

authors： Zorad S,Alsasua A,Saavedra JM

更新日期：1991-11-01 00:00:00

abstract：:A method for the destruction of a sub-population of neocortical pyramidal neurones is described. The technique uses unilateral striatal injections of volkensin, a toxic lectin from Adenia volkensii, which undergoes retrograde axonal transport from the site of injection to destroy subcortically projecting pyramidal cel...

journal_title：Journal of neuroscience methods

authors： Pangalos MN,Francis PT,Pearson RC,Middlemiss DN,Bowen DM

更新日期：1991-11-01 00:00:00

abstract：:The design of a simple and inexpensive battery powered lamp controller for microscope lamps commonly used in patch clamp and other biophysical studies is described. The circuit linearizes the normally exponential voltage-intensity curve of standard 12-V lamps by using an operational amplifier to provide feedback to th...

journal_title：Journal of neuroscience methods

authors： Galbraith JA

更新日期：1991-10-01 00:00:00

abstract：:We have developed a labeling procedure which accurately and consistently labels the original sensory pools projecting to their respective nerve branches as a model to quantify the accuracy of nerve regeneration at the single neuron level. Adult and juvenile rats had the saphenous branch of the femoral nerve transected...

journal_title：Journal of neuroscience methods

authors： Harsh C,Archibald SJ,Madison RD

更新日期：1991-09-01 00:00:00

abstract：:Threshold depolarization is defined as the amplitude of the depolarization that will just bring a neurone to its firing threshold. Here, a novel technique is described for the measurement of threshold depolarization in resting human motoneurones. The tibial nerve was stimulated at an intensity that was just sufficient...

journal_title：Journal of neuroscience methods

authors： Türker KS,Miles TS

更新日期：1991-08-01 00:00:00

abstract：:Our laboratory has previously characterized a high affinity choline uptake system (HAChUS) in Limulus tissues and synaptosomes. We report here on the characterization of the HAChUS in synaptosomes prepared selectively from central nervous system tissues shown to be enriched for presumed cholinergic functions; namely t...

journal_title：Journal of neuroscience methods

authors： Torrence-Campbell C,Gates H,Effiong U,Sweet S,Townsel JG

更新日期：1991-07-01 00:00:00

abstract：:A system for rapid solution exchange (concentration-clamp) was developed for Xenopus laevis oocytes. The exchange time (t90) was 10 ms. The system allows two-electrode voltage-clamp investigations. ...

journal_title：Journal of neuroscience methods

authors： Madeja M,Musshoff U,Speckmann EJ

更新日期：1991-07-01 00:00:00

abstract：:Digital image analysis of quantitative autoradiographic (QAR) films is widely used in neuroscience applications. Unless proper precautions are taken when autoradiographic images are converted to digital form they can be inadvertently modified by improper application of the sampling process. This type of modification i...

journal_title：Journal of neuroscience methods

authors： Lapin GD,Groothuis DR

更新日期：1991-06-01 00:00:00

abstract：:The adult mammalian central nervous system develops from the pseudostratified neuroepithelium of the neural tube. In order to study, in vitro, the differentiation of the neuroepithelial cells in detail and to identify factors that may influence this process, an uncontaminated, viable population of neuroepithelial cell...

journal_title：Journal of neuroscience methods

authors： Drago J,Murphy M,Bailey KA,Bartlett PF

更新日期：1991-05-01 00:00:00

abstract：:A new data model allowing the coding, graphic representation and metric analysis of dendritic and axonal processes including spines and varicosities, is here described. The model is implemented in an interactive light microscope-computer system and stores the three-dimensional coordinates of the selected neuronal poin...

journal_title：Journal of neuroscience methods

authors： Freire M

更新日期：1991-03-01 00:00:00

abstract：:A new neuroanatomical method based on the anterograde transport of biocytin has recently been reported. The validity of this method is examined closely on a number of well established pathways in the central nervous system, as well as the experimental parameters necessary for its effective use. iontophoretic applicati...

journal_title：Journal of neuroscience methods

authors： Izzo PN

更新日期：1991-02-01 00:00:00

abstract：:We have built a high speed, sensitive camera system capable of capturing sequences of low-light level images synchronized with recordings of membrane potential. The camera system is based on a cooled, scientific grade CCD camera controlled by a PC/AT computer. It can take 100 frames/sec of 18 X 18 element images and 4...

journal_title：Journal of neuroscience methods

authors： Lasser-Ross N,Miyakawa H,Lev-Ram V,Young SR,Ross WN

更新日期：1991-02-01 00:00:00

abstract：:This report describes a digitally controlled system for use in auditory physiology in which signal generation and data acquisition are under the control of a single microcomputer. The system is designed to generate the complex waveforms required in neuroethological studies, and is able to generate these signals at fre...

journal_title：Journal of neuroscience methods

authors： Fuzessery ZM,Gumtow RG,Lane R

更新日期：1991-01-01 00:00:00

abstract：:A method for the reconstruction of the individual spike trains from extracellular multineuron recordings is described. A neural network emulation program is trained to recognize a sample set of digitized spikes. The digitized spikes are fed into the neural network, and the network output is used to classify spikes in ...

journal_title：Journal of neuroscience methods

authors： Jansen RF

更新日期：1990-12-01 00:00:00

abstract：:The whole cell patch clamp technique allows recording of membrane currents in an entire cell under voltage clamp conditions. However, technical difficulties arise in large cells bearing extensive processes, such as human fetal dorsal root ganglion (DRG) neurons in culture. In order to improve space clamp conditions, h...

journal_title：Journal of neuroscience methods

authors： Caviedes P,Ault B,Rapoport SI

更新日期：1990-10-01 00:00:00

abstract：:Brain dialysis has become an important tool for investigating changes in the extracellular levels of neurotransmitters. This paper reviews the experimental variables and criteria that should be considered when interpreting data obtained with the brain dialysis method. Brain dialysis can provide important direct inform...

journal_title：Journal of neuroscience methods

authors： Di Chiara G

更新日期：1990-09-01 00:00:00

abstract：:Obesity-related hypertension is a clinical problem of major significance. The nature of the relationship between blood pressure and body weight has not been elucidated. Recent studies suggest that insulin (and/or insulin resistance) may be involved. An hypothesis is developed, based on the relationship between dietary...

journal_title：Journal of neuroscience methods

authors： Landsberg L

更新日期：1990-09-01 00:00:00

abstract：:The analgesic effects of various agents were evaluated in knee joint monoarthritic rats where arthritis was induced by monosodium urate (MSU) crystals injected into the knee joint cavities. The weight supported by each hind limb was measured quantitatively using pressure transducers, which revealed dose-dependent reco...

journal_title：Journal of neuroscience methods

authors： Otsuki T,Agatsuma Y,Jokura H,Sakurada S,Kisara K,Yoshimoto T

更新日期：1990-08-01 00:00:00

abstract：:The limited success of immunogold labeling for pre-embedding immunocytochemistry of neuronal antigens is largely attributed to poor penetration of large (5-20 nm) colloidal gold particles. We examined the applicability of using silver intensification of 1 nm colloidal gold particles non-covalently bound to goat anti-r...

journal_title：Journal of neuroscience methods

authors： Chan J,Aoki C,Pickel VM

更新日期：1990-08-01 00:00:00

abstract：:A method is described for the construction of simple, robust electrodes suitable for obtaining long-term chronic recordings of electromyogram activity from delicate muscles of small experimental animals. The electrodes are equipped with small, gold-plated barbed tips which have the form of harpoons that may be pushed ...

journal_title：Journal of neuroscience methods

authors： Cooke IR,Brodecky V,Berger PJ

更新日期：1990-07-01 00:00:00

abstract：:Enzymatically isolated retinal glial (Müller) cells have been the subject of many electrophysiological studies. Local high membrane conductivities for potassium ions have been speculated to correspond with local occurrence of orthogonal arrays of intramembranous particles (OAP) observed in freeze-fracture replicas of ...

journal_title：Journal of neuroscience methods

authors： Reichenbach A,Wolburg H,Richter W,Eberhardt W

更新日期：1990-06-01 00:00:00

abstract：:An inexpensive system for quantitative electroencephalogram (EEG) analysis over long time periods is described. This system continuously analyses EEG for intensity versus frequency content, and saves averaged 'data reduced' intensity spectra to disk at regular intervals. The method had adequate frequency resolution fo...

journal_title：Journal of neuroscience methods

authors： Williams CE,Gluckman PD

更新日期：1990-04-01 00:00:00

abstract：:A method for fabricating double-barrel, ion-selective microelectrodes with fine tips (0.5-1.5 microns) and rapid response times is described. When made into K(+)-selective microelectrodes, the electrodes respond to changes in [K+]o with a time constant of 70-95 ms. The electrical response of these electrodes to common...

journal_title：Journal of neuroscience methods

authors： Wen R,Oakley B 2nd

更新日期：1990-03-01 00:00:00

abstract：:While it has long been recognized that humans recover function after stroke or head injury, the direct impact of drugs on recovery has only recently received attention. The ability of rats to traverse a narrow, elevated beam has proven to be particularly useful when studying the effects of pharmacologic manipulations ...

journal_title：Journal of neuroscience methods

authors： Goldstein LB,Davis JN

更新日期：1990-02-01 00:00:00

abstract：:We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We corr...

journal_title：Journal of neuroscience methods

authors： Macklis JD,Madison RD

更新日期：1990-01-01 00:00:00

abstract：:The time course of dipeptidase activity and the effect of p-mercuribenzoate (PCMB) on the subestimation of the fluorometric determination of angiotensin-converting enzyme (ACE, EC 3.4.15.1) during development was studied. ACE and dipeptidase activities were measured fluorometrically in homogenates of the developing ch...

journal_title：Journal of neuroscience methods

authors： Nunes-Mamede ML,De Mello FG,Martins AR

更新日期：1990-01-01 00:00:00

abstract：:The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of c...

journal_title：Journal of neuroscience methods

authors： Luo XG,Hevner RF,Wong-Riley MT

更新日期：1989-12-01 00:00:00

abstract：:A system for autoregulation of body temperature of large and small animals is described. The device uses the IC AD590 as a temperature transducer. It operates on the basis of continuous regulation of the heating current, and does not emit transients which interfere with electrical recordings. A panel meter shows eithe...

journal_title：Journal of neuroscience methods

authors： Campastro OJ,Gonzalez SA,Pazo JH

更新日期：1989-12-01 00:00:00

abstract：:Serial sectioning for 3-D reconstruction requires a highly skilled and experienced individual to collect ribbons of ultrathin sections on formvar-coated grids, and to handle the grids after section collection. A simple method is described for placing ribbons in an orderly serial fashion on formvar-coated grids, by a m...

journal_title：Journal of neuroscience methods

authors： Wali N,Jagadeesh JM

更新日期：1989-11-01 00:00:00

abstract：:The use of c-fos protein (Fos) immunocytochemistry as a metabolic marker for tracing neuroanatomical connections, seizure pathways and sites of action of neuroactive drugs is discussed in this report. Fos immunocytochemistry will be very useful for these purposes providing that a number of potential problems are recog...

journal_title：Journal of neuroscience methods

authors： Dragunow M,Faull R

更新日期：1989-09-01 00:00:00